Figure 5.

Smad4 deficiency leads to defective memory. (a) The percentages and numbers of K^b-ova⁺CD8⁺ splenic T cells in Smad4^co/co;Lck-Cre mice and their littermate controls on day 35 of LM-OVA primary infection (n=6 per group). (b) The expression of CD62L and CD27 in K^b-ova⁺CD8⁺ splenic T cells of Smad4^co/co;Lck-Cre mice and their littermate controls on day 35 of LM-OVA primary infection (n=6 per group). (c) The percentages and numbers of K^b-ova⁺CD8⁺ splenic T cells in Smad4^co/co;Lck-Cre mice and their littermate controls 5 days after the secondary infection (n=6 per group). (d) Bone marrow chimeric mice were prepared and treated as described in Figure 2d. The expression of CD45.1 and OVA specificity (K^b-ova) in CD8⁺CD45.2⁺ splenic T cells at day 35 post infection was analyzed by flow cytometry (n=3). (e) Bone marrow chimeric mice were rechallenged with 1 × 10⁵ c.f.u. of LM-OVA 35 days after primary infection. The expression of CD45.1 and OVA specificity (K^b-ova) in CD8⁺CD45.2⁺ splenic T cells 5 days after the secondary infection was analyzed by flow cytometry (n=3). Data shown in this figure are representative of at least three independent experiments