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. 2015 Nov 26;6(11):e1993. doi: 10.1038/cddis.2015.338

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Copyright © 2015 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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CHBP inhibited DC maturation in vitro. BM cells from Wistar rats were plated in 24-well plates with rGM-CSF (20 ng/ml) and rIL-4 (10 ng/ml) with or without CHBP (20 nmol/l). Cells were cultured for 8 days to induce imDCs and LPS was added in the culture system for another 24 h to induce mDCs. (a) The expression of MHC-II, CD80 and CD86 on mDCs was examined by flow cytometry. (b) Levels of TNF-α, IFN-γ, IL-1β, IL-2, IL-4, IL-12 and IL-10 in the supernatant were detected using ELISA. (c) The proliferation of T cells inhibited by DCs with or without CHBP incubation was evaluated by MLR (d) Direct effect of CHBP on T cells proliferation was examined by T cells proliferation assay. Results are mean±S.D. from three independent experiments. ^#P<0.05 imDC+CHBP versus mDC, *P<0.05 imDC+CHBP versus imDC+Vehicle