Figure 2.
Apoptosis upon inhibition of Bcl-XL and Mcl-1 is mediated by Bak. (a) Primary human fibroblasts were transfected with indicated siRNAs. Protein expression was measured 48 h after siRNA treatment. Results are representative of three independent experiments. (b) Fibroblasts were treated as described in a and cell death was quantified 3 days after transfection. Data are given as means±S.D. of three independent experiments. (c) Fibroblasts were treated as described in a and activation of Bax was quantified by cytometry after staining with an antibody specific for activated Bax. Data show means±S.D. of at least three independent experiments. *P≤0.05; n.s., not significant