Figure 5.
Individual BH3-only proteins are not required for apoptosis induction upon loss of Bcl-XL/Mcl-1. (a) Primary human fibroblasts were transfected with Bcl-XL and Mcl-1 siRNA together with control or siRNA specific for Bim, Bid, Puma or Bad as indicated. Respective gene expression was analyzed 24 h after transfection. (b) Primary human fibroblasts were treated with siRNAs as indicated. Cell death was measured 3 days after transfection. (c) MEFs from Mcl-1-deficient mice were treated with two siRNAs as indicated. mRNA levels were measured 24 h after transfection. (d) Mcl-1-deficient MEFs were transfected with two BH3-only targeting or control siRNAs as indicated. Forty-eight hours after transfection, cells were transfected with Bcl-XL or control siRNAs and cell death was measured 24 h later. For a–d, data show means±S.D. of three independent experiments. *P≤0.05; ND, not determined