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. 2015 Dec 7;5:17792. doi: 10.1038/srep17792

Figure 3. CR-OSCs Transcriptionally Upregulate Hair Cell-specific Genes in Response to Differentiation Conditions.

Figure 3

(A) Primary passage CR-OSC colonies were manually harvested and plated into differentiation conditions. After 14 days the cells were fixed, stained for myosin VI (red) or Hoechst (blue), and imaged. (B) Primary passage CR-OSC colonies from Prestin-YFP mice were manually harvested and plated into differentiation conditions for 21 days after which the cells were fixed and immune-labeled by YFP antibody. (C) Primary passage CR-OSCs were differentiated for 21 days and stained for myosin VI (red), YFP (green) and Hoechst (blue). (D) Primary passage CR-OCS colonies were harvested before (0 Days) and after (14 Days) differentiation. The expression of transcripts were normalized to 18s RNA. N = 15 for all conditions. *indicates p < 0.05. Mean ± S.E.M.