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. 2015 Dec 7;5:17732. doi: 10.1038/srep17732

Figure 1. PI3K/AKT pathway promotes GSK3β Ser9 phosphorylation during the RANKL-induced Osteoclast differentiation.

Figure 1

BMM or RAW264.7 cells were treated with RANKL for the indicated durations, then analyzed by Western blotting using the indicated antibodies (a,b). (c,d) BMM were treated with RANKL alone or in combination with PI3K inhibitor LY294002 then subjected to Western blotting using the indicated antibodies or TRAP staining as described in Materials and Methods. The numbers under each protein band in the blots indicate the fold change after normalization using GAPDH and in comparison with the control group (except for DC-STAMP for which the comparisons were made with the “12 hours” group because the protein amount was obviously 0 in the control group). Gels were run under the same experimental conditions. For better clarity and conciseness of the presentation, cropped blots are shown. The raw uncropped images can be found in the Supplementary Figure 1. The cropping lines are indicated by black lines both in cropped and uncropped images. The results shown are representative of at least 3 experiments.