Figure 2. Developmental EE2 exposure did not alter reproductive physiology.

EE2 exposure had no effect on plasma testosterone concentrations of F1 to F4 males (A), or on adult body weight (B), ano-genital distance (AGD) (C), testis weight (D) or seminal vesicles/coagulating glands (SV/CG) weight (E) of F1 to F3 males (n = 3–5 litters for each group of each generation) (Kruskal-wallis’ test). Each parameter measured was reported to the animal’s body weight to be expressed as an index of animal body weight. Since no significant alterations in reproductive tract morphometric parameters were detected, F4 males were not investigated for these analyses.