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. 2015 Dec 7;5:17470. doi: 10.1038/srep17470

Figure 2. Enhanced SRF activity causes aberrant cell positioning and arborization in the developing cortex.

Figure 2

(A) Immunostaining against SRF indicates that SRF is expressed during cortex development. Scale bar: 100 μm. (B) Left panel: Scheme of the in utero electroporation experiment. Center panels: composite images of coronal brain sections of P20 mice electroporated at E14.5 with GFP alone (GFP) or in combination with caSRF or ciSRF. Brain slices were stained with anti-GFP antibody and counterstained with DAPI. Scale bar: 500 μm. Right panels: Four phenotypes associated with caSRF expression: (a) impaired migration of some neurons to the cortical layers. (b) aberrant layer positioning, (c) misoriented apical dendrite, (d) axon mistargeting along corpus callosum. Scale bar: 100 μm. (C) Left panels: Higher magnification images of electroporated neurons at the ipsilateral side after triple staining with DAPI (DNA counterstaining), anti-GFP and anti-MAP2 (neuronal dendritic marker). Central and right panels: Detail of GFP staining at the ipsi- and contralateral sides. Scale bar: 100 μm.