Figure 8. Chromosomal localization of the SRY gene on metaphases, interphase nuclei and spermatozoa using Fluorescence in situ hybridization (FISH).

(a) The metaphase and interphase nuclei are stained with DAPI. The SRY gene localized on the Y chromosome is showing red signal and X-centromere, fluorescent green signal. (b) Figure represents mapping of SRY gene on the individual sperm. All the Y bearing sperms are shown in red, whereas those of X-bearing ones are shown in green. (c) Male blood metaphase and (e) sperm samples were processed under identical conditions but not hybridized with the SRY probe to exclude the background signal. (d) Female sample hybridized with SRY probe. The spermatozoa are stained with DAPI. Patient IDs are shown in yellow. AZ indicates azoospermic; OS, oligospermic; INS, infertile males with normal spermiogram and NFM, normal fertile male.