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. 2015 Dec 7;5:16683. doi: 10.1038/srep16683

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Representative serial sections of cholesteatomas from CC and AC patients were used to analyze pathological profiles (n = 12). Congenital cholesteatoma (A) acquired cholesteatoma (B) and eroded bone from acquired cholesteatoma patients (C) were stained with hematoxylin and eosin, as described in the Materials and Methods section. The images in panels (A–C) were obtained using a 20× objective. Black arrows indicate lamellar sheets of keratin and an incrassated epithelium, and the large black asterisk in (C) indicates an osteoclast.