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. 2015 Dec 7;5:16683. doi: 10.1038/srep16683

Table 5. Sequences of Primers used in the PCR.

Gene Oligonucleotide sequence (5′-3′)
Human β-actin (F) GCTCCTCCTGAGCGCAAG
Human β-actin (R) CATCTGCTGGAAGGTGGACA
Human TLR2(F) CCGTAGATGAAGTCAGCTCACCGATG
Human TLR2(R) CCTCCGACAGTTCCAAGA TGT AACGC
Human TLR4(F) GAGGACCGACACCAATGATG
Human TLR4(R) GAACGAATGGAATGTGCAACACC
Human TLR5(F) TGCTCAAACACCTGGATGCTCACTAC
Human TLR5(R) ACAGCCGCCTGGATGTTGGAGATATG
Human TLR9(F) ACCTTCCATCACCTGAGCCATCTG
Human TLR9(R) GCCGCTGAAGTCAAG AAACCTCAC
Human TNF-α (F) CCAGGCAGTCAGATCATCTTC
Human TNF-α (R) GTTATCTCTCAGCTCCACGC
Human IL-1β (F) CGCAGCAGCACATCAACAAGAGC
Human IL-1β (R) TGTCCTCATCCTGGAAGGTCCACG
Mouse β-actin (F) GAT TAC TGC TCT GGC TCC TAG C
Mouse β-actin (R) GAC TCA TCG TAC TCC TGC TTG C
Mouse TNF-α (F) CACAGAAAGCATGATCCGCGAC
Mouse TNF-α (R) TGCCACAAGCAGGAATGAGAAGAG
Mouse IL-1β (F) CGC AGC AGC ACA TCA ACA AGA GC
Mouse IL-1β (R) TGT CCT CAT CCT GGA AGG TCC ACG
Mouse IL-10(F) AGCTGGACAACATACTGCTAACCGAC
Mouse IL-10(R) CTTGATTTCTGGGCCATGCTTCTCTG
Mouse iNOS (F) CTA AGA GTC ACC AAA ATG GCT CCC
Mouse iNOS (R) AGA CCA GAG GCA GCA CAT CAA AGC
Mouse BD-2(F) TCT CTG CTC TCT GCT GCT GAT ATG C
Mouse BD-2(R) AGG ACA AAT GGC TCT GAC ACA GTA CC
Mouse-TRAP (F) CGC-ACA-GGT-AGGCAG-TGA-C-
Mouse-TRAP (R) CTA-CCC-CGT-GTGGTC-CAT-AG-
Mouse-RANKL (F) TAACCCTTAGTTTTCCGTTGC
Mouse-RANKL (R) CCTGAGACTCCATGAAAACGC
Mouse-OPG (F) TCCTGGCACCTACCTAAAACAGCA
Mouse-OPG (R) CTACACTCTCGGCATTCACTTTGG