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. 2015 Dec 7;5:17804. doi: 10.1038/srep17804

Figure 1.

Figure 1

(a) Schematic illustration for the photoswitching of the Cy3-Alexa647 heterodimer. The ‘reporter’, Alexa647, emits the fluorescence upon a red laser illumination during which it forms a dark state, whereas the ‘activator’, Cy3, helps the activation process under green laser irradiation. (b) Fluorescence emission showing the on/off cycles upon repetitive irradiation under optimized illumination condition (activation: 532 nm, 0.5 ms, 1.6 kW/cm2; depletion: 633 nm, 15 ms, 4.0 kW/cm2) and imaging buffer (143 mM of MEA and 100 mM of KI). (c) Photobleaching behavior of Cy3-Alexa647 in imaging buffer and Atto532 in PVA.