Figure 3. FACS analysis of DiO-HDL fluorescence and radiolabeled cholesteryl [¹⁴C]-oleate HDL uptake in 24, 48 and 96 h cultured 3T3 Swiss mouse fibroblasts.

Panel a: shows the FACS diagram of fluorescence signal related to DiO-HDL uptake in 24, 48 and 96 h fibroblasts after contact loss. Cells were incubated with DiO-HDL and prepared for FACS analysis as reported in Material and Methods section. Each experiment was carried out at least twice and the relative graph shows the weighted mean value ± SE of fluorescence evaluated in positive cells (P2) normalized to the entire population (P1 + P2). At least 10000 cells for each group were analysed. *p < 0.01 vs 24 h (t-student test, unpaired two-tailed). Panel b: cells were incubated with 100 mM [¹⁴C]-CE-HDL in LPDS 24 h before the harvesting times. Cells were harvested and lipids extracted as described in Material and Methods section. Each experiment was carried out at least twice in triplicate and the results are given as mean ± SE. *p < 0.05 vs all (t-student test, unpaired two-tailed).