Figure 6. Cell cycle distribution, SR-BI, AKT and ERK protein expression in serum-deprived cells entering the cell cycle after 24 h treatment with FCS, HDL or PDGF.

Sub-confluent cells were cultured for 24 h in serum-deprived medium (SDC). Then treated for further 24 h with FCS, HDL or PDGF, respectively. At the end of experiments cells were harvested and processed as required for cell cycle analysis by FACS (a) and western blotting (b), respectively. For details see Materials and Methods. The panel (c–g) show the densitometric analysis of SR-BI, AKT, pAKT, ERK and pERK bands, respectively. Data were reported as mean ± SE of three different experiments. *p < 0.05 vs SDC; °p < 0.05 vs FCS; ^p < 0.05 vs HDL (ANOVA, Fischer’s LSD as post hoc test).