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. 2015 Dec 7;5:17809. doi: 10.1038/srep17809

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(a) The polymorphonuclear characteristic of human primary neutrophils was determined with Giemsa staining. (b) The expression of CD15 on separated cells was analyzed with immunofluorescence. The primary antibody was mouse anti-CD15, the secondry antibody was Alexa Fluor 555 conjugated goat anti-mouse (red), and the nuclei were stained with DAPI (blue). (c) Flow cytometry was performed to depict the purity of neutrophils with PE-conjugated anti-CD15 antibody (blue curve). PE-conjugated mouse IgM was used as the isotype control (red curve). Scale bar, 20 μm.