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Nutrient-restricted or ILP/IIS-deficient flies show severe growth defect. Wild-type flies, wild-type adult female flies raised either on a nutrient-rich diet (Well-fed) or low-protein diet (Nutrient-restricted). ILP/IIS-deficient flies, a brain mNSC-ablated female fly (DILP-producing mNSCs in the brain were genetically ablated using a dilp2 promoter to express the pro-apoptotic gene, reaper) and an InR hypomorphic mutant female fly.
