Fig. 2.

Poly-ICR induces HPV16-exposed LC to upregulate CCR7 and migrate to CCL21 in vitro. LC were exposed to HPV16 prior to s-Poly-I:C (Poly-ICR) treatment as described. (A) CCR7 expression was analyzed by flow cytometry. Data represent mean fold increase in CCR7 expression (±SEM) relative to untreated LC based on MFI (N=10 individual donors). (B) In vitro migration assay. LC were analyzed for migration through a transwell insert to CCL21 or medium alone. Shown is the mean number of LC migrating to CCL21 (black bars) compared to spontaneous migration (± SEM) relative to untreated LC (N=6 individual donors). ^⁎⁎p<0.01 compared to untreated LC and LC exposed to HPV16 (one-way ANOVA, Tukey׳s post-test).