Fig. 6.

Poly-ICLC induces LC activation in HPV16-exposed LC. (A) Poly-ICLC induces upregulation of MHC and costimulatory molecules on LC. LC were left untreated or exposed to HPV16 prior to treatment with s-Poly-I:C (50 μg/mL Poly-ICLC), then analyzed by flow cytometry for indicated surface markers. Control cells were left untreated or exposed to HPV16 alone. Data shown is the mean fold increase in surface marker expression (±SD) of replicate values relative to untreated LC based on MFI of one representative donor out of four tested. ^⁎p=0.05, (Mann–Whitney U test). (B) Poly-ICLC induces HPV16-exposed LC to migrate to chemokine CCL21 in vitro. LC were exposed to HPV16 prior to s-Poly-I:C (50 μg/mL Poly-ICLC). LC were analyzed for migration to medium or medium supplemented with CCL21. Shown is the mean number (±SD) of LC migrating to CCL21 (black bars) compared to spontaneous migration (white bars) of three separate donors performed in triplicate relative to untreated LC. *p=0.05 compared to HPV16 only LC (Mann–Whitney U test).