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. 2015 Nov 9;195(12):5592–5601. doi: 10.4049/jimmunol.1501929

FIGURE 1.

FIGURE 1.

IdeS cleaves IgG-type, but not IgM-type, of BCR on B cells. (A) Flow cytometry analysis of cells stained with biotinylated anti-Fab Ab, followed by streptavidin-allophycocyanin, after treatment of Nu-DUL-1 cells (IgG-type) and Daudi cells (IgM-type) with the indicated concentrations of IdeS. The y-axis shows MFI of duplicate samples in FL4, and vertical lines represent SD. The data are representative of two independent experiments. (B) Flow cytometry analysis of Nu-DUL-1 cells (IgG-type) stained with either biotin-conjugated anti-Fab or anti-Fc, followed by streptavidin-allophycocyanin, after treatment with the indicated concentrations of IdeS. The y-axis shows MFI in FL4. The data are representative of two independent experiments.