FIGURE 3.

Recovery of surface BCR expression on B cells after IdeS treatment. (A) Flow cytometry analysis of the presence of F(ab′)₂ fragments on the surface of Nu-DUL-1 cells after treatment with different amounts of IdeS. IdeS was removed, and cells were cultured and analyzed after 1 and 24 h. Cells were stained with biotin-conjugated anti-Fab Ab, followed by streptavidin-allophycocyanin. The y-axis shows relative fluorescent intensity for which non-IdeS–treated signals were assigned 100%. The data are representative of two independent experiments. (B) Nu-DUL-1 cells were treated with different amounts of IdeS or antiproliferative control substances (cytochalasin D and puromycin) and cultured for 24 h prior to a 6-h BrdU incorporation. Data are mean ± SD of triplicate samples. The data are representative of two independent experiments. (C) Nu-DUL-1 cells were treated with PBS or IdeS (30 μg/ml) for 24 h before an intracellular hydrogenase activity–based viability assay (CCK-8) was used as read-out. The data are representative of two independent experiments.