Table 1. Physiologic measurements before, during, and after ischemia and stereotactic injection.
| Sham-shSRCmiR | Sham-shCDK5miR | Ischemia-SRCmiR | Ischemia-CDK5miR | ||
|---|---|---|---|---|---|
| Before surgery (15 minutes) | |||||
| Arterial blood pressure(mm Hg) | 66.8±2.64 | 64.3 ±1.37 | 69.2±3.09 | 71.7±1.14 | n.s. |
| pH | 7.3±0.01 | 7.3± 0.02 | 7.3±0.04 | 7.3±0.04 | n.s. |
| pCO2 (mm Hg) | 55.6±2.42 | 53.2±1.79 | 53.1±5.75 | 49.3±5.75 | n.s. |
| pO2 (mm Hg) | 267.1±32.85 | 261.8±32.15 | 225.2±38.88 | 220.1±38.88 | n.s. |
| Glucose (mg/dL) | 306.8±19.46 | 332.2±34.69 | 305.2±29.10 | 295.5±8.47 | n.s. |
| Lactate (mmol/L) | 0.7±0.17 | 0.9±0.12 | 0.9±0.11 | 0.9±0.36 | n.s. |
| Hematocrit | 39±2.073 | 41±0.71 | 39.7±0.86 | 41.7±1.03 | n.s. |
| Sodium (mmol/L) | 148.6±4.89 | 146±2.43 | 144.75±0.25 | 147.5±3.84 | n.s. |
| Potasium (mmol/L) | 4.3±0.31 | 4.6±0.14 | 4.2±0.01 | 4.2±0.11 | n.s. |
| Calcium (mmol/L) | 1.3±0.09 | 1.3±0.03 | 1.4±0.02 | 1.3±0.06 | n.s. |
| Temperature (ºC) | 34.2±0.12 | 34.5±0.38 | 34.15±0.56 | 33.875±0.43 | n.s. |
| During surgery (15 minutes) | |||||
| Arterial blood pressure (mm Hg) | 76.7±6.63 | 74.7±1.42 | 89.7±3.34* | 89.4±2.27* | P=0.04 |
| pH | 7.3±0.02 | 7.3±0.018 | 7.4±0.01* | 7.4±0.01* | P=0.026 |
| pCO2 (mm Hg) | 55.1±3.19 | 51.5±2.63 | 42.8±1.94* | 38.9±1.94* | P=0.042 |
| pO2 (mm Hg) | 268.8±36.29 | 271.5±37.68 | 236.9±33.21 | 230.6±33.21 | n.s. |
| Glucose (mg/dL) | 310.2±28.65 | 321.4±28.91 | 325.2±34.66 | 296±19.01 | n.s. |
| Lactate (mmol/L) | 1.034±0.19 | 0.85±0.20 | 0.93±0.17 | 1.04±0.08 | n.s. |
| Hematocrit | 42.2±0.73 | 42.2±1.28 | 41.5±1.66 | 42.5±1.55 | n.s. |
| Sodium (mmol/L) | 142.2±0.49 | 142.2±1.32 | 142.5±0.29 | 143.5±2.10 | n.s. |
| Potasium (mmol/L) | 4.76±0.19 | 4.58±0.14 | 4.35±0.13 | 4.37±0.15 | n.s. |
| Calcium (mmol/L) | 1.4±0.04 | 1.4±0.04 | 1.3±002 | 1.3±0.06 | n.s. |
| Temperature (ºC) | 34.14±0.58 | 34.46±0.10 | 33.9±0.49 | 33.7±0.32 | n.s. |
| After surgery (15 minutes) | |||||
| Arterial blood pressure (mm Hg) | 80.6±8.73 | 80.3±6.60 | 86.3±1.78 | 89.7±5.178 | n.s. |
| pH | 7.3±0.03 | 7.3±0.02 | 7.3±0.01 | 7.3±0.01 | n.s. |
| pCO2 (mm Hg) | 52.1±5.68 | 51.9±3.1577 | 45.1±1.77 | 45.4±1.77 | n.s. |
| pO2 (mm Hg) | 273.7±19.86 | 254±28.15 | 242.1±24.05 | 268.6±24.05 | n.s. |
| Glucose (mg/dL) | 306.8±20.35 | 290.2±29.27 | 305±26.51 | 283.8±9.49 | n.s. |
| Lactate (mmol/L) | 0.8±0.20 | 0.9±0.27 | 1±0.15 | 1±0.13 | n.s. |
| Hematocrit | 41.6±0.75 | 42.2±0.8 | 41.2±2.35 | 41.7±0.63 | n.s. |
| Sodium (mmol/L) | 142.6±0.68 | 143±1.26 | 141.7±0.75 | 142.5±1.32 | n.s. |
| Potasium (mmol/L) | 4.7±0.23 | 4.8±0.12 | 4.5±0.17 | 4.5±0.11 | n.s. |
| Calcium (mmol/L) | 1.4±0.019 | 1.4±0.03 | 1.3±0.06 | 1.3±0.05 | n.s. |
| Temperature (°C) | 33.46±0.273 | 34.22±0.239 | 33.45±0.67 | 33.25±0.466 | n.s. |
Values are mean±s.e.m., SHAM: Simulation (Control), ISCHEMIA: t-MCAO, transient middle cerebral artery occlusion.
The data are expressed as the group mean±s.e.m. *Significant differences: *P<0.05; n=10 to 12 animals/group.