Figure 5. Transgenic overexpression of STC1 diminishes thrombin protein and activity in the lung after bleomycin treatment.

One week after intra-tracheal administration of bleomycin, lungs were harvested, homogenized, and assayed for thrombin protein abundance and activity as described in methods. (A) The proteolytic activity of thrombin is expressed in relative fluorescent units. Values represent means ± SEM (n = 5). (B,C) Transgenic overexpression of STC1 diminishes thrombin protein expression. WT or STC1 Tg mice were treated with either saline or BLM. One week later, lungs were harvested, homogenized, protein lysates were resolved on SDS-PAGE, and Western blots reacted with anti-thrombin or anti-GAPDH. (B) Representative blots are shown. (C) Bar graph depicts band intensities for thrombin normalized to GAPDH; data represent the mean± SEM (n = 6–10).