Figure 1. Immunofluorescence analysis of PDGFRα expression in implanted sponge.

(a) Immunofluorescence of PDGFRα (green) and PDGFRβ (red) in Flox mice (upper row) and α-KO mice (bottom row) with nuclear staining (Hoechst, blue). (b–d) Multicolor immunofluorescence analysis of PDGFRα expression in Flox mice. Co-staining of PDGFRα (green) and CD31 (red; b), PDGFRα (green) and αSMA (red; c), PDGFRα (green) and F4/80 (red, d). The nuclei were stained with Hoechst (blue, b–d). PDGFRα was not expressed in endothelial cells, including specific tip cells, vascular smooth muscle cells/pericytes, and macrophages, all of which are major components of ingrowing connective tissue together with fibroblasts. Scale bars indicate 10 μm, respectively.