Table 1. Primer sequences and amplicon characteristics of the 10 candidate internal control genes evaluated in this study.
| Gene | A. thaliana ortholog locus | Gene description | Primer sequence F/R (5′–3′) | Product size (bp) | Efficiency (%) | Mean | CV (%) | ||
|---|---|---|---|---|---|---|---|---|---|
| R 2 | Ct | SD | |||||||
| 18S rRNA | At3g41768 | 18S ribosomal RNA | CTACGTCCCTGCCCTTTGTA | 175 | 104.1 | 0.9949 | 17.89 | 1.546 | 8.64 |
| GGTTCACCTACGGAAACCTTG | |||||||||
| ACT4 | At5g09810 | Actin 4 | TTGCAGACCGTATGAGCAAG | 166 | 105.4 | 0.9967 | 22.17 | 1.240 | 5.59 |
| ATCCTCCGATCCAGACACTG | |||||||||
| MZA | At5g46630 | Clathrin adaptor complexes | CCGTCAGACAGATTGGAGGT | 154 | 106.3 | 0.9949 | 34.28 | 0.7416 | 2.16 |
| medium subunit family protein | AAAGCAACAGCCTCAACGAC | ||||||||
| EF1α | At5g60390 | Elongation factor 1-alpha | TCCCCATCTCTGGTTTTGAG | 130 | 113.8 | 0.9960 | 22.46 | 1.542 | 6.87 |
| CTTGGGCTCATTGATCTGGT | |||||||||
| RAN | At5g59840 | Ras-related small | GCCATGCCGATAAGAACATT | 167 | 97.13 | 0.9997 | 32.18 | 1.151 | 3.58 |
| GTP-binding protein | GTGAAGGCAGTCTCCCACAT | ||||||||
| TUBα | At4g14960 | Alpha-tubulin | AATGCTTGCTGGGAGCTTTA | 213 | 105.1 | 0.9992 | 30.24 | 0.6500 | 2.15 |
| GTGGAATAACTGGCGGTACG | |||||||||
| PP2A | At1g59830 | Catalytic subunit of protein | GATCCTTGTGGAGGAGTGGA | 201 | 108.9 | 0.9985 | 28.38 | 1.149 | 4.05 |
| phosphatase 2A | GCGAAACAGTTCGACGAGAT | ||||||||
| UBC | At3g52560 | Ubiquitin-conjugating | CTGCCATCTCCTTTTTCAGC | 150 | 118.6 | 0.9981 | 23.72 | 1.169 | 4.93 |
| enzyme like protein | CGAGTGTCCGTTTTCATTCA | ||||||||
| PTB | At3g01150 | Polypyrimidine tract-binding | GGTTACCATTGAGGGTGTGG | 158 | 109.4 | 0.9993 | 28.14 | 1.321 | 4.69 |
| protein homolog | GTGCACAAAACCAAATGCAG | ||||||||
| UBQ | At5g20620 | Ubiquitin | TCTTTGCAGGGAAGCAACTT | 219 | 102.5 | 0.9993 | 31.15 | 1.944 | 6.24 |
| CTGCATAGCAGCAAGCTCAC | |||||||||