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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Mol Microbiol. 2015 Jul 4;97(5):881–897. doi: 10.1111/mmi.13073

Fig. 6. Rv0888 enables utilization of sphingomyelin cleavage products as nutrient sources.

Fig. 6

Phosphocholine utilization of M. smegmatis SMR5 (wt), the M. smegmatis porin mutant ML16 (ΔmspA, ΔmspC, ΔmspD) and ML16 expressing mspA, rv0888 or rv0888*. Ten-fold serial diluted cultures were dropped on (A) low-phosphate HdB/kan plates with regular amounts of phosphate (12 mM), no phosphate (trace phosphorus) and HdB plates with trace amounts of phosphorus supplemented with 0.5 mM phosphocholine. (B) Diluted cells were transferred on low-nitrogen HdB/kan plates with regular amounts of ammonium sulfate (15 mM), no ammonium sulfate (trace nitrogen) and HdB plates with trace amounts of nitrogen supplemented with 20 mM phosphocholine. The concentration of phosphocholine in these experiments was determined empirically. Lanes: 1, SMR5/pMS2kan (wt, empty vector); 2, ML16/pMS2kan (empty vector); 3, ML16/pML632 (+mspA); 4, ML16/pML2118 (+rv0888); 5, ML16/pML3108 (+rv0888*).