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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Mol Microbiol. 2015 Jul 4;97(5):881–897. doi: 10.1111/mmi.13073

Fig. 7. Expression of rv0888 enhances intracellular replication of M. tuberculosis in macrophages.

Fig. 7

Differentiated THP-1 macrophages were infected with Mtb ML1528 (H37Rv L5::pCV125; wt), ML1566 (rv0888::loxP), ML923 (rv0888::loxP, L5::rv0888) and ML925 (rv0888::loxP, L5::rv0888*) at an MOI of 1. Bacterial strains were grown in HdB medium supplemented with 0.75 mM sphingomyelin and 0.2% glucose to logarithmic phase before infection. Macrophages were lysed after 4 h and 5 days post-infection and the number of viable bacteria was counted as colony forming units (CFU) on agar plates. Mean values of biological triplicates are shown with standard deviations. The data was logarithmically transformed to achieve normal distribution and a one-way ANOVA in combination with a Tukey test was performed. The p values are * (p < 0.05); ** (p < 0.01); *** (p < 0.001).