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. Author manuscript; available in PMC: 2015 Dec 7.
Published in final edited form as: Nature. 2015 Mar 23;521(7553):541–544. doi: 10.1038/nature14328

Extended Data Figure 6. Rev7 loss promotes end resection at DSBs in BRCA1-deficient cells after IR.

Extended Data Figure 6

a, Quantification of RPA positive alpha tracks in KB1P-B11 cells 1h or 2h after irradiation with alpha particles. b, Quantification of RPA and 53BP1-positive alpha tracks in KB1P-B11 cells transfected with non-targeting control siRNAs or siRNAs against CtIP. c, Cell cycle analysis (BrdU incorporation and propidium iodine labeling) of KB1P-B11 cells transduced with the indicated constructs and siRNAs. . d, e, Quantification of Rev7 transcript (d) or protein (e) levels in BRCA1-deficient mES cells transduced with Rev7-targeting shRNAs or the vector control. Hprt was used as a control for transcript expression. The data represent the mean ± SD. f, Representative images of surviving colonies of Brca1−/− mES cells transduced with an empty vector control or Rev7-targeting shRNAs. g, Quantification of colony formation normalized to the vector control. h, Quantification of RAD51 foci in Brca1−/− mES cells that were transduced with the indicated constructs.