Extended Data Figure 2. Loss of REV7 causes olaparib resistance in BRCA1-deficient SUM149PT cells.
a, Western Blotting analysis of REV7 or 53BP1 expression in SUM149PT cells transduced with REV7- or 53BP1-targeting hairpins or the vector control. b, Example of a long-term clonogenic survival assay using the indicated hairpins and olaparib concentrations. c, Quantification of the clonogenic assays using absorbance of crystal violet at 590nm. The data represent the mean ± SD. All the groups were normalized by the absorbance of the vector control and showed significant differences to the control (P< 0.01, t-test).