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Construction of MV gates using size-calibrated fluorescent beads. (A) Selection of bead subsets using FACSAria instrument with a set SSC-H threshold of 200, and determination of small- (<0.5 μm) and large-size (>0.5 μm) gates. The solid square is the total MV gate and the dotted line separates the small- and the large-size gates. (B) Application of the MV gates to a plasma sample.
