Figure 1.

Schematic Representation of RNA Interactome Capture. Living cell monolayers are irradiated with UV light to covalently link direct protein–RNA interactions. Polyadenylated RNA and covalently bound protein partners are isolated by oligo(dT) pull-down under denaturing conditions. After RNase treatment, the RNA-binding protein (RBP) repertoire is determined by quantitative mass spectrometry, comparing proteins isolated from crosslinked cells (cCL) with those present in a mock pull-down (noCL). Only proteins with consistent enrichment across replicates (encircled in red) are considered as the RNA interactome.