Table 1. An outline of evidences that vmi480 and vmi470 constitute a TA module.
Aspects | Evidences |
---|---|
Genetic analysis | 1. vmi480 and vmi470 constitute an operon with their own promoter and their genetic configuration resemble those of most type II TA systems. |
2. vmi470 contains a DNA-binding helix-turn-helix domain shared by several antitoxins. | |
Deletion mutants | 3. Deletion of vmi480 or vmi480-vmi470 can be achieved while deletion of sole vmi470 cannot fulfill. |
Ectopic expression | 4. Sole expression of vmi480 is lethal to the cells. |
5. Sole expression of vmi470 has no effect on cell growth. | |
6. Lethality of Vmi480 can be eliminated when both genes are co-expressed in one vector. |