|
E. coli
|
|
|
| DH5α λpir
|
F− φ80 lacZ ΔM15 recA1 end A1 hsdR17 supE44 thi-1 gyrA96 relA1 (lacZYA-argF) U169 λpir lysogen |
Laboratory collection |
| NEB5α |
|
NEB |
| β2163 |
(F-) RP4-2-Tc::Mu ΔdapA::(erm-pir) |
[21] |
| LP79 |
MG1655 MGIVmi1 res2::pSW23T (NxR, CmR) |
[22] |
| LP86 |
MG1655 MGIVmi1 res2::pSW23T pKD46 (NxR, CmR, ApR) |
This study |
| LP116 |
MG1655 MGIVmi1 res2::pSW23T Δvmi480-470 (NxR, CmR) |
This study |
| LP128 |
MG1655 MGIVmi1 res2::pSW23T Δvmi480 (NxR, CmR) |
This study |
| LP134 |
NEB5α pBAD30-vmi480-470 (ApR) |
This study |
| LP135 |
NEB5α pBAD30-vmi470 (ApR) |
This study |
| LP192 |
NEB5α pBAD30-vmi480 (ApR) |
This study |
| LP194 |
DH5α λpir pLP10 (CmR) |
This study |
| LP196 |
DH5α λpir pLP11 (CmR) |
This study |
| LP197 |
DH5α λpir pLP12 (CmR) |
This study |
|
V. alginolyticus
|
|
|
| E0601 |
Isolated from seawater in Yangjiang, China |
This study |
| LP204 |
E0601 hem::pLP11 (CmR) |
This study |
| LP206 |
E0601 hem::pLP12 (CmR) |
This study |
| LP207 |
E0601 Δhem (resulted from LP204) |
This study |
| LP208 |
E0601 Δhem (resulted from LP206) |
This study |
|
V. cholerae
|
|
|
| HN375 |
Isolated from seawater in Zhanjiang, China |
This study |
| LP228 |
HN375 degS::pLP12 (CmR) |
This study |
| LP233 |
HN375 ΔdegS
|
This study |
| LP230 |
HN375 vasC::pLP12 (CmR) |
This study |
| LP235 |
HN375 ΔvasC
|
This study |
|
V. parahaemolyticus
|
|
This study |
| E0680 |
Isolated from an oyster in Yangjiang, China |
This study |
| E06135
|
Isolated from Litopenaeus vannamei in Yangjiang, China |
This study |
| LP246 |
E0680 pilO:: pLP12 (CmR) |
This study |
| LP250 |
E0680 ΔpilO
|
This study |
| LP248 |
E06135 ascS:: pLP12 (CmR) |
This study |
| LP252 |
E06135 ΔascS
|
This study |
|
V. vulnificus
|
|
This study |
| ATCC 27562 |
|
ATCC |
| LP239 |
ATCC 27562 impB:: pLP12 (CmR) |
This study |
| LP244 |
ATCC 27562 ΔimpB
|
This study |
| Plasmids |
|
|
| pSW23T |
oriT
RP4
oriV
R6K (CmR) |
[21] |
| pSW25T-ccdB
|
oriT
RP4
oriV
R6K
ccdB (SpR) |
Laboratory collection |
| pBAD30 |
ori
p15A
araC PBAD (ApR) |
[40] |
| pKD4 |
PCR template for one-step gene inactivation (KnR) |
[25] |
| pKD13 |
PCR template for one-step gene inactivation (KnR) |
[25] |
| pKD46 |
λ- recombination plasmid (ApR) |
[25] |
| pCP20 |
Helper plasmid to delete resistant gene with FRT sites (ApR) |
[25] |
| pLP10 |
oriT
RP4
oriV
R6K
ccdB PTAC promoter (CmR) |
This study |
| pLP11 |
oriT
RP4
oriV
R6K
vmi480 PTAC promoter (CmR) |
This study |
| pLP12 |
oriT
RP4
oriV
R6K
vmi480 PBAD (CmR) |
This study |
| pLP11-hem
|
pLP11 containing homologous arms of hem gene of E0601 |
This study |
| pLP12-hem
|
pLP12 containing homologous arms of hem gene of E0601 |
This study |
| pLP12-degS
|
pLP12 containing homologous arms of degS gene of HN375 |
This study |
| pLP12-vasC
|
pLP12 containing homologous arms of vasC gene of HN375 |
This study |
| pLP12-pilO
|
pLP12 containing homologous arms of pilO gene of E0680 |
This study |
| pLP12-ascS
|
pLP12 containing homologous arms of ascS gene of E06135
|
This study |
| pLP12-impB
|
pLP12 containing homologous arms of impB gene of ATCC 27562 |
This study |
