Fig 1. Nucleotide and amino acid sequences of pre-pro-TcCYSPR04.

Nucleotides of ORF and non-coding regions are shown in uppercase and lowercase letters, respectively. Signal peptide is underlined. White letters on dark gray background indicate the pro-region of the protein. The light gray region represents the catalytic region of the protein. Putative phosphorylated amino acids are indicated in boxes. The double underline shows the putative glycosylated amino acids. ■ indicates the vacuolar targeting motif SNPIR. ▲ and • indicates the ERFNIN and ANFD motifs of the pro-region. Amino acids from the catalytic site are indicated by *. Peptides identified by mass spectrometry after capture by TcCYS4 immobilized on CNBr-sepharose resin are indicated. ♦ indicates the stop codon.