Fig 5. Protease activity of protein captured by TcCYS3 and TcCYS4 coupled to CNBr–Sepharose.

Qualitative assay to test protease activity of samples obtained by capturing system. a SDS-PAGE gel showing bands of protease activity of about 30 and 45 kDa (arrows). b IEF gel showing clear bands of protease activity at about pH 4.0. BSA—sample captured by BSA-CNBr–Sepharose (control). TcCYS3—sample captured by recombinant TcCYS3–CNBr–Sepharose. TcCYS4—sample captured by recombinant TcCYS4–CNBr–Sepharose.