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. 2015 Dec 7;10(12):e0144441. doi: 10.1371/journal.pone.0144441

Fig 2. Knockdown of NDUFB9 promoted MDA-MB-231 proliferation.

Fig 2

(A) Knockdown efficiency of NDUFB9 in MDA-MB-231 and MCF-10A cells analyzed by Western blot and the below panel shows histograms of the results. (B) CCK-8 proliferation assays in MDA-MB-231 shCon and shNDUFB9 cells. (C) Cell cycle analysis in MDA-MB-231 shCon and shNDUFB9 cells at 6h and 12h after cells were synchronized at the G1/S boundary. (D, E) Histograms of the S and G2/M phases of the cell cycle for 6 h and 12 h respectively. Results above are repeated at least 3 times. Data were presented as the mean ± SD (Student’s t-test, n≥3; *P < 0.05, **P < 0.01 and ***P < 0.001).