Fig 3. Loss of NDUFB9 expression resulted in enhancement of MDA-MB-231 cell migration and invasion.

(A) The migration and invasion ability of MDA-MB-231 and migration of MCF-10A were evaluated with a transwell assay. The left panels show images of representative fields (100× magnification) of invasive cells, and the right panel shows histograms of the results. (B) Effects of NDUFB9 on the migration of MDA-MB-231 cells in a wound-healing assay. The red lines indicate the initial scratch wound location, and the yellow area shows the scratch wound mask. Images were captured at the indicated times after wounding. (C) The effect of NDUFB9 on the percent of relative wound density at the indicated time point. Results above are repeated at least 3 times. Data were presented as the mean ± SD (Student’s t-test, n≥3; **P < 0.01, and ***P < 0.001).