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. 2015 Dec 7;10(12):e0144441. doi: 10.1371/journal.pone.0144441

Fig 4. Depletion of NDUFB9 altered mitochondrial metabolism.

Fig 4

(A) FACS analysis (left) and statistical data (right) of mitochondrial ROS generation in MDA-MB-231 and MCF-10A cells. (B) Intracellular NAD+, NADH, total NAD+/NADH and the ratio of NAD+/NADH were analyzed independently in extracts from whole cells in the NDUFB9 knockdown and control groups. (C) mt-DNA content was determined by quantitative real-time PCR of mtDNA extracted from MDA-MB-231 cells, using three pairs of mtDNA primers (primer-1, primer-2, and primer-3). (D, E) Glucose uptake and lactate secretion were assessed in NDUFB9 knockdown or control MDA-MB-231 cells. Results above are repeated at least 3 times. Data were presented as the mean ± SD (Student’s t-test, n≥3; **P < 0.01, and ***P < 0.001).