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. 2015 Dec 7;10(12):e0144441. doi: 10.1371/journal.pone.0144441

Fig 5. Suppression of NDUFB9 expression induced EMT and activated the AKT/mTOR/p70S6K signaling pathway.

Fig 5

(A-B) Immunoblotting analysis of E-cadherin, Fibronectin-1 (FN-1), Vimentin, phosphorylated SMAD3, SMAD3 and SMAD4 in MCF-10A and MDA-MB-231 cells transfected with NDUFB9-specific or control shRNA. GAPDH was used to normalize for equal loading. (C) Expression levels of phosphorylated Akt (S129), total Akt, phosphorylated mTOR(S2448), total mTOR, phosphorylated p70S6K and p70S6K were examined by immunoblotting in MDA-MB-231. Results above are representative of 3 independent experiments. (D-F) Histograms of the results from A, B and C respectively. Data were presented as the mean ± SD (Student’s t-test, n≥3; **P < 0.01, and ***P < 0.001).