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. 2015 Dec 7;10(12):e0144508. doi: 10.1371/journal.pone.0144508

Fig 6. Upregulation of annulate lamellae causes a redistribution of both pore complexes and nuclear transport receptors from the nuclear envelope to annulate lamellae.

Fig 6

(A) HeLa cells were treated with vinblastine or DMSO as a control and analyzed by immunofluorescence microscopy with mAb414 and anti-RanGAP1 antibodies. (B-F) HeLa cells were transfected with control or ELYS-specific siRNAs, double-labeled with mAb414 and anti-ELYS antibodies (B), mAb414 and anti-RanGAP1 antibodies (C), anti-importin α and anti-RanGAP1 antibodies (D), anti-importin β and anti-RanGAP1 antibodies (E), and anti-CRM1 and anti-RanGAP1 antibodies (F) followed by immunofluorescence microscopy. The boxes at the corner of each image represent the enlarged version of inlets. Bar, 10 μm.