Skip to main content
. 2015 Dec 7;10(12):e0144508. doi: 10.1371/journal.pone.0144508

Fig 10. RanBP2 is required for the localization of RanGTP and CRM1 at NPCs and ALPCs.

Fig 10

(A) HeLa cells were first transfected with control or RanBP2-specific siRNAs for 48 h and then the constructs encoding FLAG-tagged RanQ69L for 24 h followed by immunofluorescence microscopy with antibodies specific to RanBP2 and FLAG. (B) HeLa cells were transfected with control or RanBP2-specific siRNAs for 72 h and then analyzed by immunofluorescence microscopy with antibodies specific to RanBP2 and CRM1. In RanBP2 RNAi cells (lower panels), “-” indicates the cell with depletion of RanBP2, and “+” indicates the cell with levels of RanBP2 that are similar to those in control cells (upper panels). The boxes at the bottom corner of each image show the enlarged version of inlets. Bar, 10 μm.