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. 2015 Dec 7;10(12):e0144347. doi: 10.1371/journal.pone.0144347

Fig 1. PEA modification of Gc lipid A enhances Gc survival during association with murine macrophages.

Fig 1

(A): Schematic presentation of the lipid A structures of Gc strains with or without PEA modification. The PEA moiety containing a positively charged amino group is circled in blue and negatively charged phosphate groups are circled in red. PEA is absent from the lipid A of the lptA::spec mutant [16]. (): Percent survival of the total Gc inoculum in association with RAW264 macrophage culture was assessed after 2 hrs of phagocytosis. Murine RAW264 macrophages were infected with live WT (FA19) or mutant (lptA::spec) at an MOI of 50 (n = 3). After one hour of phagocytosis, macrophages were washed extensively to remove extracellular Gc that are not tightly bound to cells. Survival of Gc associated with macrophages was assessed after 2 hrs of phagocytosis using standard plating and CFU counts. p values were calculated using Student t-test in reference to cells infected with the WT (*) strain. These data are representative of three independent experiments.