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. 2015 Dec 7;10(12):e0144495. doi: 10.1371/journal.pone.0144495

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© 2015 Zhu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — Immunoblotting analysis was performed to analyze the effects of high glucose (HG) and 100 ng/mL bFGF on phosphorylation levels of JNK and ERK respectively (A, B, E, F) during 72 hours of period and effects of 30 minutes of bFGF stimulation before HGstressed human VECs for 72 hours. All experiments were performed after exposure of cells to 5 mg/mL mitomycin C (to inhibit proliferation) for 1 day. (C), (D), (G), (H), (K), (L) Results are presented as fold changes relative to LG-incubated VECs. Data represent mean values ± SE of four independent experiments, relative to the LG group (*P < 0.05) and the HG group (^# P < 0.05, ^## P < 0.01).