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. 2015 Dec 7;10(12):e0144495. doi: 10.1371/journal.pone.0144495

Fig 6. High-glucose increases generation of reactive oxygen species (ROS) in VECs.

Fig 6

(A) The probe 2’,7’-dichlorohydrofluorescein (DCFH2-DA) was added to the medium of cells treated with low glucose (LG) or high glucose (HG). HG-treated cells were also treated with 100 ng/mL bFGF, 1 μM sp600125 (sp), or 1 μM U0126 (U). ROS were observed in all cells, especially in high glucose-treated cells. Bar = 100 μm. (B) Fluorescence levels in ten different cells were measured using the Image Pro Plus software. Data represent mean values ± SE of four replicates, relative to the LG group (***P < 0.001) and relative to the HG group (# P < 0.05, ## P < 0.01).