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. 2015 Dec 7;10(12):e0144495. doi: 10.1371/journal.pone.0144495

Fig 8. High glucose-induced accumulation of intracellular ROS levels delays VEC migration.

Fig 8

(A) Cells were pretreated with high glucose (35 mM) for 72 hours, and then incubated with the ROS scavenger MnTmPyP (10 μM) for 1 hour. Intracellular ROS levels were measured using DCFH-DA dye. Bar = 100 μm. (B) Wound scratch assay was performed to analyze the effects of 10 μM MnTmPyP in human VECs. LG: medium containing 5.5 mM glucose. Bar = 500 μm. All experiments were performed after exposure of cells to 5 mg/mL mitomycin C (to inhibit proliferation) for 1 day. (C) Fluorescence levels in ten different cells were measured using the Image Pro Plus software. Data represent mean values ± SE of four replicates, relative to the LG group (***P < 0.001) and relative to the HG group (###P < 0.001). (D) Cell migration distance was determined according to the data shown in (B). Data represent mean values ± SE of ten replicates, relative to the HG group (*P < 0.05, **P < 0.01).