Table 3. Quantification of RSV replication via RT-qPCR and infectious focus assay in lambs inoculated with M37 hRSV.
| Viral load (Nasal Washes)* | Viral load (BALF)* | Viral transcripts(Lung tissue) | |||
|---|---|---|---|---|---|
| Viral culture (Log10 FFU/mL ± se) | RT-qPCR (Log10 M37 RNA copies/mL ± se) | Viral culture (Log10 FFU/mL ± se) | RT-qPCR (Log10 M37 RNA copies/mL ± se) | RT-qPCR (Log10 M37 RNA copies/mg lung tissue ± se) | |
| Day 1 | BDL (0.7) | 1.44 ± 1.1 | 2.53 ± 0.09 | 3.80 ± 0.03 | 4.81 ± 0.15 |
| Day 3 | 1.7 ± 0.4 | 1.68 ± 1.4 | 4.60 ± 0.32 | 6.22 ± 0.08 | 6.51 ± 0.08 |
| Day 4 | 0.6 ± 0.1 | 0.42 ± 0.1 | 3.94 ± 0.25 | 6.20 ± 0.16 | 6.67 ± 0.25 |
| Day 6 | 0.99 ± 0.3 | 2.89 ± 0.34 | 4.83 ± 0.04 | 7.15 ± 0.2 | 7.63 ± 0.07 |
| Day 8 | 0.8 ± 0.1 | 1.46 ± 1.2 | 1.02 ± 0.32 | 4.70 ± 0.38 | 5.24 ± 0.26 |
*BDL = below detection limit. All samples BDL were assigned a value of 0.7 log10 FFU/mL for culture and 0.3 log10 RNA copies/mL for RT-qPCR. Values in brackets depict the standard error.