Figure 1.

Inflammatory index. Cultures of human aortic endothelial cells were treated with culture medium alone (No Additions), with low-density lipoprotein (LDL) from a normal healthy individual (Std LDL), or with LDL plus high-density lipoprotein (HDL) from a normal healthy individual (Std LDL + Std HDL). After overnight incubation, cultures were washed, fresh medium was added, incubation continued, and the supernatant was assayed in a standard chemotaxis chamber, as described in “Methods.” A, NCB (No Cell Blank) indicates the values for the number of monocytes migrated toward culture supernatant without incubation with endothelial cells. B, Data were calculated by subtracting the NCB values. The inset shows the inflammatory index for HDL (HII) by taking the value for Std LDL+ Std HDL and dividing it by that for Std LDL.