Figure 3.

Mutant bone morphogenetic protein receptor II (BMPR2) mice had a similar level of lung fibrosis following bleomycin compared to control, with increased collagen content. a, Representative microscopy images of trichrome-stained lung sections of groups as listed, showing no difference in lung architecture at baseline and similar fibrosis after exposure to bleomycin. Magnification ×20. b, Graph representing no difference in control versus BMPR2 groups with respect to fibrosis score before and after intraperitoneal bleomycin treatment (P = 0.90). c, However, there was significantly increased collagen content in the right lower lobe of harvested lungs by microplate hydroxyproline assay in the bleomycin BMPR2 group compared to control. PBS: phosphate-buffered saline. Asterisk indicates P < 0.05. N = 8 per group, except N = 4 for control vehicle.