Table 1.
Gene primers sequences for mRNA amplification
| Gene name | Forward (5′–3′) | Reverse (5′–3′) | Product length |
|---|---|---|---|
| TNF-α | AAGGGAATTGTGGCTCTGGG | ACTCAGGCATCGACATTCCG | 179 |
| IL-6 | AGAGACTTCCAGCCAGTTGC | ACAGTGCATCATCGCTGTTC | 232 |
| IL-1β | GCCAACAAGTGGTATTCTCCA | CCGTCTTTCATCACACAGGA | 118 |
| Cox-2 | GTTGCTGGGGGAAGGAATGT | AGAAGCGTTTGCGGTACTCA | 112 |
| TGF-β | AGGGCTACCATGCCAACTTC | CCACGTAGTAGACGATGGGC | 168 |
| α-SMA | ACCATCGGGAATGAACGCTT | TTGCGTTCTGGAGGAGCAAT | 263 |
| Colla1 | GCAATGCTGAATCGTCCCAC | CAGCACAGGCCCTCAAAAAC | 176 |
| Colla2 | TAAAGAAGGCCCTGTGGGTC | ATGGCCTTTCTCACCAGGTTT | 148 |
| TIMP-1 | ACGCTAGAGCAGATACCACG | CCAGGTCCGAGTTGCAGAAA | 141 |
| MMP-1 | AACAGCAGAAGATTTCCCTGG | TTCGGAGGCTAAATCTGCGT | 110 |
| Desmin | CCGATCCAGACCTTCTCTGC | TCTCCATCCCGGGTCTCAAT | 116 |
| GAPDH | CAGGGCTGCCTTCTCTTGTG | GGTGGTGAAGACGCCAGTAG | 256 |