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. Author manuscript; available in PMC: 2016 Dec 3.
Published in final edited form as: Mol Cell. 2015 Nov 12;60(5):728–741. doi: 10.1016/j.molcel.2015.10.012

Figure 2. A L1 3′ poly(A) tract is required for retrotransposition.

Figure 2

A. Schematic of L1 retrotransposition assay: The pAT2-MALAT expression plasmid contains retrotransposition indicator (mneoI) and MALAT cassettes. Retrotransposition leads to an L1 insertion at a new genomic DNA location (gray bars). Expression of NEO protein (purple oval) confers G418-resistance to HeLa cells.

B. Results from retrotransposition assay: Schematics of engineered L1 constructs (left) and results of retrotransposition assay (right). X-axis: construct names and G418-resistant foci from a representative experiment. Y-axis: relative retrotransposition efficiency. pAT2-SV, positive control (set at 100%). pAD135 construct (an RT- allele), negative control. Error bars, standard deviation in a representative experiment containing three technical replicates. Three biological replicates were performed for each experiment. See also Figure S2 and Table S1.