Fig. 1.

Molecular analysis of selected primary OsCKX2 transformants (T0) and wild-type (WT) plants. a Detection of transgenes in selected primary transformants. The presence of CX3 (or CX5), hptII and GUS from selected primary transformants was detected by PCR. M: DNA markers, P: positive control (plasmid DNA as template), WT: untransformed wild type, N: negative control (H₂O as template). b Expression of OsCKX2 in selected primary transformants and wild type (WT), as assayed by RT-PCR using specific primers. Expression of actin was used as a cDNA loading control